Search results for "Wash water"

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Application of propidium monoazide-qPCR to evaluate the ultrasonic inactivation of Escherichia coli O157:H7 in fresh-cut vegetable wash water.

2012

The efficacy of sanitizing technologies in produce or in vegetable wash water is generally evaluated by plate count in selective media. This procedure is time consuming and can lead to misinterpretations because environmental conditions and sanitizing processes may affect bacterial growth or culturable capability. Thus, the aim of this study was to determine the applicability of a propidium monoazide real-time PCR (PMA-qPCR) method to monitor the inactivation by ultrasound treatment of foodborne bacteria in fresh-cut vegetable wash water. To this aim, lettuce wash water was artificially inoculated with Escherichia coli O157:H7 (10⁶ CFU/mL) and treated by means of a continuous ultrasonic irr…

AzidesCell SurvivalFood HandlingColony Count MicrobialFood ContaminationBiologyBacterial growthmedicine.disease_causeEscherichia coli O157Real-Time Polymerase Chain ReactionMicrobiologyMicrobiologyPropidium monoazideVegetablesmedicineFood scienceEscherichia coliDetection limitFoodborne bacteriabiology.organism_classificationDisinfectionWash waterConsumer Product SafetyFood MicrobiologyUltrasonic sensorBacteriaFood SciencePropidiumFood microbiology

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